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KMID : 0358320060470020189
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Korean Journal of Urology
2006 Volume.47 No. 2 p.189 ~ p.194
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The Protein Expressions of Apoptosis-associated Genes in the Obstructed Ureters of Rats
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Kim Hyeong-Gon
Paick Sung-Hyun
Lho Yong-Soo
Kim Hyeon-Hoe
Kwak Cheol
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Abstract
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Purpose: The role of apoptosis in the pathogenesis of ureteral damage from obstructive uropathy has rarely been studied. This study was performed to determine the protein expression of the apoptosis-associated genes in the pathogenesis of ureteral damage during the course of obstructive uropathy in ligated rat ureters.
Materials and Methods: After unilateral ligation of the ureter, each group of five Sprague-Dawley rats was sacrificed and examined at 1, 5, 10, 15, 20, 25, 30 and 35 days after ligation: five rats with normal ureters were also examined as controls. The protein expressions of the fas-associated death domain(FADD), Bax, Bcl-xL and cyclooxygenases(COX)-2 genes in obstructed ureters were assessed by performing Western blotting.
Results: The expressions of FADD protein in the 20 and 25 day-obstructed ureters(DOUs) were significantly higher than that in control ureters and the peak was reached in the 25 DOUs. The expressions of Bcl-xL protein in the 20, 25 and 30 DOUs were significantly higher than that in the control ureters and the peak was reached in the 25 DOUs. The expression of COX-2 protein in the 5, 10, 15, 25 DOUs were significantly higher than that in the control ureters and the peak was reached in the 10 DOUs.
Conclusion: The FADD and Bcl-xL genes were involved in apoptosis of the obstructed ureter. The peaks of their expressions were at 25 DOUs. The expression of the COX-2 gene may be related with apoptosis in the obstructed ureter. (Korean J Urol 2006;47:189-194)
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KEYWORD
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Ureteral obstruction, Apoptosis, Rats
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